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Reduced levels of free IAA are seen in combination mutants defective in IBA metabolism and IAA-conjugate hydrolysis; 5-d-old cotyledon tissue was collected, and free IAA levels were measured using gas chromatography-mass spectrometry. n ≥ 6; error bars represent se. a to c, Significant differences using one-way ANOVA (P < 0.05). f.w., Fresh weight.
