Figure 2.

Inhibition of auxin-regulated gene expression by OGs and elf18 is not affected in anp triple mutants. Expression of the indicated auxin-regulated genes in the wild type (Ws) and amiR1 (line 2.5) and amiR3 (line 5.3) mutant seedlings grown in the presence of β-estradiol. Seedlings were treated for 1 h with water (mock), OGs, elf18, IAA (1.5 µm), IAA plus OGs, or IAA plus elf18, as indicated. Analyses were performed by qRT-PCR, and transcript levels are shown as the mean of at least three independent experiments (± se; n = 20 in each experiment) normalized to UBQ5 expression and plotted relative to expression in mock-treated Ws. Asterisks indicate statistically significant differences between cotreatment with IAA plus elicitor and treatment with IAA alone according to Student’s t test (**P < 0.01). In inset, levels of IAA5 transcripts in mock-treated seedlings are shown.