Figure 5.

anp single and double mutant plants are defective in elicitor-induced protection against B. cinerea. Protection conferred by pretreatment with water (H; control), OGs (O), or elf18 (E) was determined in anp single (A) and double (B) mutant plants, in transgenic anp3 (C) and anp2 anp3 double (D) mutant plants overexpressing ANP3, and in corresponding wild-type plants. Different letters above bars indicate statistically significant differences (P < 0.05) between samples, as determined by ANOVA with Tukey’s honestly significant difference mean-separation test. The experiments were repeated three times with similar results (n = 20 in each experiment). E, Expression of the PAD3 gene was determined by qRT-PCR in adult Col-0 and anp single mutant leaves 6 h after spraying with water, OGs, or elf18. Analyses were performed by qRT-PCR, and transcript levels are shown as the mean of at least three independent experiments (± se; n = 5 in each experiment) normalized to UBQ5 expression and plotted relative to expression in mock-treated Col-0. Asterisks indicate statistically significant differences between elicitor-treated mutants and corresponding treatment of the wild type according to Student’s t test (P < 0.01). F, Amount of camalexin measured in infected leaves of Col-0 and anp single mutant plants pretreated with water or OGs at 18 h after B. cinerea inoculation. Mean values (± se; n = 4) are shown. Asterisks indicate statistically significant differences between OG and water pretreatment according to Student’s t test (P < 0.01). The experiment was repeated three times with similar results.