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The elicitor-induced oxidative burst is defective in anp mutants. ROS production was measured using a luminol-based assay in leaf discs from anp double mutant (A) and amiR1 and amiR3 triple mutant (B) plants and in wild-type plants after elicitation with water, OGs, or elf18. Results are mean ± se (n = 12). Experiments were repeated at least three times with similar results.
