Figure 9.

MPK3 and MPK6 phosphorylation is defective in anp mutant seedlings in response to OGs but not to elf18. Levels of phosphorylated MAPKs (pMPK3 and pMPK6) after elicitation with OGs or elf18 in the wild type (Ws), anp1 anp2 and anp2 anp3 double mutants, and amiR1 and amiR3 anp triple mutants were determined by immunoblot analysis using an antip44/42-ERK antibody (top). Levels of MPK3 and MPK6 total proteins were determined using specific antibodies (bottom). The identity of individual MAPKs as determined by size is indicated by arrows. Experiments were repeated at least three times with similar results.