Figure 4.

Transcriptional regulation of the ZFP3 gene. A, qRT-PCR analysis of ZFP3 expression during germination. Wild-type seeds were germinated in the absence (Control) or presence of 2 μm ABA (ABA). GAPC2 (At1g13440) was used as a reference transcript. Relative transcript values are shown, where 1 equals transcript levels at the start of germination (Fohgrub and Kempken, 2012). ΔΔCT, Delta delta cycle threshold. B to O, Detection of pZFP3-GUS activity in transgenic Arabidopsis. Histochemical localization of GUS activity is shown in developing and mature seeds (B and C), germinating seeds (D and E), 3-d-old seedlings germinated in light (F) or dark (G), 7-d-old plantlets (H–K), and flowers (L–O) of transgenic plants. Bars = 0.1 mm (B–E, J, K, N, and O) and 1 mm (F–I, L, and M).