Figure 3.
KAI2 and AtD14 differentially mediate responses of multiple transcripts to SL stereoisomers. Seed were sown, stratified, and incubated as described in Figure 2, and RNA was isolated from whole seedlings after 4 d. Two transcripts known to respond positively to rac-GR24 (STH7 and DLK2) and two that respond negatively (IAA5 and IAA6) were measured. Target transcripts were normalized to CLATHRIN ADAPTOR COMPLEX SUBUNIT reference transcripts, and expression was scaled relative to Col-0 grown on one-half-strength Murashige and Skoog (MS). Data are means ± ses of three biological replicates of >100 seedlings each. Asterisks denote significant differences between selected treatments as indicated (ANOVA); for clarity, not all significant differences are shown. *, P < 0.05; **, P < 0.01.