Figure 5.

ABA levels in dry seeds and seed germination rates after the fluridone treatment. A, ABA levels in dry seeds of Col-0 and cer9-2 measured using the Phytodetek-ABA kit. FW, Fresh weight. B, Seeds of Col-0 and cer9-2 were immersed in water or 100 µm fluridone at 4°C for 48 h. The treated seeds were washed three times with water and planted on 1/2 MS agar plates supplemented with 0, 1, or 2 µm ABA. Seed germination was scored after 2 d. Seeds were considered germinated when the radicles emerged. *P < 0.05. Col-0 (+F) and cer9-2 (+F) represent Col-0 and cer9-2 with fluridone treatment, respectively.