Figure 7.

Immunogold detection of VSRs in wild-type and transgenic Arabidopsis plants with induced expression of ESCRT-III mutants. Six-day-old wild-type plants (A and B) and transgenic plants (C–J) germinated and cultured in the absence of inducers were transferred onto medium containing 10 μm β-estradiol for 24 h to induce the expression of VPS20.1-1-112-YFP (C and D), SNF7.1(L22W)-YFP (E and F), VPS24.1-1-152-YFP (G and H), or VPS2.1-1-179-YFP (I and J). Arabidopsis roots were subjected to high-pressure frozen/freeze substitution followed by ultrathin sectioning and immunogold labeling using VSR antibodies. Bar = 100 nm.