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. 2014 May 16;165(3):1367–1379. doi: 10.1104/pp.114.242404

Figure 8.

Figure 8.

AtZAT6 binds to the promoters of pathogen-related genes and CBF genes. A, Schematic diagrams of the effectors (35S-vector and 35S-AtZAT6) and reporters (m35S-GUS, TACAAT-m35S-GUS, and TAAAAT-m35S-GUS) used for transient expression analysis. B, Transient expression of representative tobacco leaves infiltrated with different combinations of effector and reporter. 35S::GFP plastid was coinjected as an internal standard in each infection. C, Schematic structure of the TACAAT motifs in the promoter of pathogen-related genes and CBF genes. The relative positions were chosen for ChIP-PCR analysis. D, ChIP enrichment to show the in vivo binding ability of 35S::GFP-ZAT6 to the DNA fragments in the promoters of pathogen-related genes and CBF genes. The ChIP results were normalized to input chromatin, and a fragment in the ACTIN2 promoter was used as the negative control. The data represent the means ± sds of three independent experiments, and asterisks indicate the significant difference of P < 0.05. [See online article for color version of this figure.]