Figure 1.

AtLPLA and AtLIP2 are mitochondrial enzymes expressed in leaves and roots. A, Protein-stained SDS gel (top) with 10 µg of protein per lane of leaf (le), root (ro), and mitochondrial matrix proteins (mi), and immunoblots (bottom) treated with antibodies raised against recombinant AtLPLA, AtLIP2, H-protein (mitochondrial control), and the large subunit of Rubisco (as a plastidial control). Lane M shows size-marker proteins. B, RT-PCR using total RNA from roots (ro), rosette leaves (le), cauline leaves (cl), stems (st), siliques (si), and flowers (fl). Transcripts of the constitutively expressed 40S ribosomal protein S16 were used for calibration. Oligonucleotide primers are listed in Supplemental Table S1. Stars indicate alternative splicing.