Figure 4.

Immunoblots of RNAi lines with reduced contents of LPLA and LIP2. Ten micrograms of leaf and 5 µg of root proteins were separated on SDS-polyacrylamide gels and blotted onto polyvinylidene difluoride membranes. Antibodies used were directed against Arabidopsis LPLA (anti-LPLA), Arabidopsis mitochondrial octanoyltransferase (anti-LIP2), LA epitopes of lipoylated E2 proteins and H-protein (anti-LA), and a fragment of Arabidopsis mitochondrial apoPDH-E2a (At3g52200; anti-PDH-E2a). PDH-E2 variants were named as described by Taylor et al. (2002). Numbers and letters represent individual RNAi lines. WT, Wild type.