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Cytoplasmic columns are smaller in cesa5-1 sos5-2 seed coat epidermal cells. Columella development in cryo-fixed (A to H) and glutaraldehyde-fixed (I–L) resin-embedded seed coat epidermal cells stained with toluidine blue. A to D, 8 DPA. E to H, 10 DPA. I to L, Mature seeds. A, E, and I, Col-0. B, F, and J, cesa5-1. C, G, and K, sos5-2. D, H, and L, cesa5-1 sos5-2. C, Columella; cc, cytoplasmic column; m, mucilage; mp, mucilage pocket. Bar = 10 μm.
