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. 1999 Mar 15;103(6):843–849. doi: 10.1172/JCI4217

Figure 4.

Figure 4

The SP antagonist CP-96,345 inhibits NT-mediated mucosal mast cell degranulation in rat colon. Colonic explants (2 × 2 mm) were cultured at 37°C in Krebs buffer alone or in buffer containing 0.1 mM of either the SP receptor antagonist CP-96,345 or the NT receptor antagonist SR-48,692. After 1 h, either NT or SP (10–8 M) was added to the explants, and mucosal mast cell degranulation was determined after 2 h by measuring RMCPII released into the culture media. Both SP and NT caused a significant mast cell degranulation as measured by increased levels of RMCPII in the culture media. NT-induced mast cell degranulation was completely inhibited by the SP receptor antagonist CP-96,345, but not by its inactive enantiomer, CP-96,344. Data are presented as mean ± SEM of four experiments, with triplicate determinations per group. **P < 0.01 vs. control; ++P < 0.01 vs. NT alone. SP, substance P.