Table I.

Primer sequences and length of PCR product.

PCR type	Primer name	Primer sequences	Product length, bp
qPCR	PTCH1	5′-TGTGCGCTGTCTTCCTTCTG-3′ 5′-ACGGCACTGAGCTTGATTC-3′	119
	β-actin	5′-GCCATCCTGCGTCG-3′ 5′-TGGGCACCGGAACCGCT-3′	260
MSP	Methylation	5′-GTTAATTCGTGATTTTTCGGA-3′ 5′-ATAACAAACCTACGAACCGC-3′	197
	Unmethylation	5′-AATGTTAATTTGTGATTTTTTGGA-3′ 5′-TAAATAACAAACCTACAAACCAC-3′	197

qPCR, quantitative PCR; MSP, methylation-specific PCR.