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5-FU induces caspase-9-dependent apoptosis. HCT116 and SW480 cells were treated with 5-FU (50 μM) for the indicated time points (0, 16, 24 and 36 h). Whole cell lysates were subjected to western blot analysis and western blot analysis of the GAPDH levels was included to demonstrate that equivalent amounts of protein were loaded in each lane. Data are representative of two individual experiments. 5-FU, 5-fluorouracil; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
