Figure 5.

Effect of MACC1 on c-MET. (A) Luciferase activity of c-MET. OVCAR3 cells were co-transfected with a recombinant plasmid and MACC1-siRNA or a negative control. Luciferase assays were performed 24 h following gene transfection. The relative luciferase activity was defined as the luciferase value, normalized to Renilla levels. (B) Protein was extracted from the MACC1 silencing group and control groups, and c-MET protein levels were measured by western blot analysis. GAPDH was used as a loading control. ^*P<0.05. MACC1, metastasis-associated in colon cancer 1; NC, negative control; siRNA, small interfering RNA; luc, luciferase.