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. 2014 May 26;8(2):775–780. doi: 10.3892/ol.2014.2174

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Copyright © 2014, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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CD40 downregulation correlates with MDSC accumulation during tumor progression. (A) Primary tumor growth was evaluated by tumor size (tumor length and width) every two to three days. The results of three independent experiments are shown. (B) CD40 expression on tumor-infiltrating MDSCs was observed with tumor progression. Representative examples are shown from three independent experiments. The correlation between MDSC accumulation and tumor progression or CD40 expression was assessed using Pearson’s correlation analysis. (C) Similar results were obtained from splenocytes. MDSCs, myeloid-derived suppressor cells; PE, phycoerythrin; PC, phycoerythrin-cyanine; CD, cluster of differentiation.