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. 2014 May 26;8(2):775–780. doi: 10.3892/ol.2014.2174

Figure 3.

Figure 3

CD40-induced apoptosis regulates MDSC accumulation. (A) Splenocytes and tumor tissue cells obtained from tumor-bearing mice were incubated with PBS or agonistic anti-CD40 for 24 h and stained with anti-Gr-1, anti-CD11b and Annexin V. Data are presented as the percentage of Annexin V+ cells in gated Gr-1+/CD11b+ MDSCs. (B) Proportion of Annexin V+/Gr-1+/CD11b+ cells from tumors and spleens expressed as the percentage of total Gr-1+/CD11b+ cells. Data are presented as the mean ± standard error of the mean (n=3 per group). *P<0.05 vs. control. MDSCs, myeloid-derived suppressor cells; PBS, phopshate-buffered saline; CD, cluster of differentiation; FS, forward scatter; SS, side scatter.