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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Traffic. 2014 Jan 22;15(3):292–308. doi: 10.1111/tra.12146

Figure 5. Particle tracking data of Rab11a labeled particles in live HeLa cells.

Figure 5

A) Time lapse, widefield movies of mCherry-Rab11a particles were collected using a Deltavision deconvolution microscope. Images were taken every 2 seconds for 2 minutes total. Track speed mean and track displacement length were calculated in at least 11 cells for each condition. A) mCherry-Rab11a vesicle speed increased significantly from 0.27 ± 0.001 μm/s in cells expressing wild type Rab11-FIP2 to 0.34 ± 0.001 μm/sin the presence of the Rab11-FIP2(S229P/G233E). Rab11a track displacement length increased significantly from 5.50 ± 0.04 μm to 6.57 ± 0.03 μm in wild type-expressing cells vs. Rab11-FIP2 mutant cells. B) mCherry-Rab11a vesicle speed increased significantly from 0.28 ± 0.001_μm/s in scrambled control to 0.33 ± 0.001 μm/s Rab11-FIP2 knockdown cells. For rescue, mCherry-Rab11a vesicle speed decreased from 0.33 ± 0.001 μm/s in Rab11-FIP2 knockdown cells to 0.27 ± 0.001_μm/s in Rab11-FIP2 knockdown cells plus Venus-Rab11-FIP2 wild type. Vesicle speed increased from 0.27 ± 0.001 μm/s in Rab11-FIP2 knockdown plus wild type to 0.31 ± 0.001 μm/s in knockdown plus Rab11-FIP2(S229P/G233E) mutant. mCherry-Rab11a track displacement length increased significantly from 4.63 ± 0.036 μm in scrambled control to 6.42 ± 0.039 μm in Rab11-FIP2 knockdown cells. mCherry-Rab11a displacement significantly decreased from 6.42 ± 0.039 μm in Rab11-FIP2 knockdown plus Venus-Rab11-FIP2 wild type to 4.56 ± 0.029 mm in Rab11-FIP2 knockdown alone. Track displacement length in Rab11-FIP2 knockdown plus Venus-Rab11-FIP2(S229P/G233E) was significantly increased from 4.56 ± 0.029 mm to 5.58 ± 0.026 μm in Rab11-FIP2 knockdown plus Venus-Rab11-FIP2. Results are presented as the mean ± SEM. * p<0.001