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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Traffic. 2014 Jan 22;15(3):292–308. doi: 10.1111/tra.12146

Figure 6. Particle tracking distribution of Rab11a labeled vesicle speed and distance in live HeLa cells.

Figure 6

mCherry-Rab11a vesicles were tracked by Imaris software in Figure 6 and data was graphed as distributions. X axis labels in B,D, and F denote Bin Centers which are the midpoints of each category. A) Track displacement length was distributed over 0-55 μm and grouped into 5 μm bins. Chi square analysis was done over the four groups (Scrambled control, Rab11-FIP2 knockdown, Rab11-FIP2 knockdown + Venus-Rab11-FIP2, Rab11-FIP2 knockdown + Venus-Rab11-FIP2(S229P/G233E)) with p<0.0001 across all groups. B) Track displacement length bar graph of mCherry-Rab11a vesicles moving 5 μm distance or less in each of the 4 conditions. Z-test for statistical significance between populations was calculated and * = p<0.001. C) Track speed mean was distributed over 0-1.3 μm/sec and grouped into 0.05 μm/sec bins. Chi square analysis was done over the four groups (Scrambled control, Rab11-FIP2 knockdown, Rab11-FIP2 knockdown + Venus-Rab11-FIP2, Rab11-FIP2 knockdown + Venus-Rab11-FIP2(S229P/G233E)) with p<0.0001 across all groups. D) Track speed mean bar graph of mCherry-Rab11a vesicles moving 0.25 μm/sec speed or less in each of the 4 conditions. Z-test for statistical significance between populations was calculated and * = p<0.001. E) Vesicle speed (instantaneous) was distributed over 0-2.78 μm/sec and grouped into 0.05 μm/sec bins. Chi square analysis was done over the four groups (Scrambled control, Rab11-FIP2 knockdown, Rab11-FIP2 knockdown + Venus-Rab11-FIP2, Rab11-FIP2 knockdown + Venus-Rab11-FIP2(S229P/G233E)) with p<0.0001 across all groups. F) Vesicle speed (instantaneous) bar graph of mCherry-Rab11a vesicles moving 0.05μm/sec speed or less in each of the 4 conditions. Z-test for statistical significance between populations was calculated and * = p<0.001.