Table 1.
Summary of Shotgun Bisulfite Sequencing
| Sample | Stage | Genome Depths |
BS Conversion Rate |
Number of CG (1×) |
CG (1×) Covered |
Number of CG (5×) |
CG (5×) Covered |
|---|---|---|---|---|---|---|---|
| Sperm | gamete | 31 | 99.53% | 22.0 M | 95.18% | 20.6 M | 89.25% |
| Egg | 35 | 99.73% | 22.1 M | 95.93% | 21.0 M | 90.89% | |
| 16 cell | cleavage | 19 | 99.44% | 21.6 M | 93.76% | 18.9 M | 82.07% |
| 32 cell | 32 | 99.89% | 22.1 M | 95.69% | 20.8 M | 90.19% | |
| 64 cell | 19 | 99.34% | 20.8 M | 90.19% | 17.8 M | 77.25% | |
| 128 cell | blastula | 22 | 99.51% | 21.9 M | 95.00% | 19.9 M | 86.14% |
| 1,000 cell | 38 | 99.53% | 22.0 M | 95.45% | 20.8 M | 90.15% | |
| Germ ring | gastrula | 20 | 99.57% | 21.7 M | 94.09% | 19.3 M | 83.41% |
| Testis | organ | 21 | 99.44% | 22.0 M | 95.27% | 20.1 M | 87.03% |
Paired reads were mapped uniquely to the reference genome (Zv9, UCSC) by Bismark. Number of CpG (1×) or number of CpG (5×) indicate total number of CpG sites mapped at least one read or five reads. Covered indicates proportion of mapped CpG sites over total CpG sites in genome. “M” indicates million.