Expression of pCICAM-1 and pCLFA-3 on human rhabdomyosarcoma (RD) cells in vitro. RD cells were transfected with pCDNA3 (control), pCICAM-1, or pCLFA-3. Three days after transfection, the cells were removed from the plates and were analyzed by FACS® analysis using either control antibodies, α–intracellular adhesion molecule-1 (ICAM-1), or α–lymphocyte function associated-3 (LFA-3) antibodies to detect expression of the transfected gene product.