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. Author manuscript; available in PMC: 2014 Jul 4.
Published in final edited form as: Anat Rec (Hoboken). 2012 Oct 8;295(11):1896–1908. doi: 10.1002/ar.22586

Figure 1.

Figure 1

Whole-mounts of the basal cochlear auditory epithelium stained for neurofilament (red) and actin (green), viewed with epifluorescence microscopy. (a) 90 days following deafening via administration of 5% neomycin directly into the scala tympani of the cochlea, all hair cells and supporting cells are absent and replaced by a flat epithelium. Neurofilament positive peripheral auditory fibers are absent with the exception of a few looping fibers extending into the basilar membrane area. (b and c) Three months after inoculation of a deafened ear with AAV.NT3 (b) and AAV.BDNF (c), there is a persistent flat epithelium with no regeneration of supporting cells or hair cells. However, there are copious neurofilament positive fibers extending in a radial fashion into the basilar membrane area. Most of the fibers weave between the junctions of the flat epithelial cells. Scale Bar=50 µm.