TABLE 3.
Summary of PAS kinase substrates reported from studies of yeast and hPASK
| Organism | Substrate | Evidence | Study |
|---|---|---|---|
| S. cerevisiae | Ugp1 UDP-glucose Pyrophosphorylase |
In vitro kinase assay with Psk2 PSK-dependent in vivo phosphorylation UGP1-S11A mutant mimics psk1−psk2− mutant |
(22,37,39) |
| Caf20 Cap Associated Factor |
In vitro kinase assay with Psk2 | (39) | |
| Tif11 (eIF1A) Translation Initiation Factor |
In vitro kinase assay with Psk2 | (39) | |
| Sro9 Suppressor of Rho3 |
In vitro kinase assay with Psk2 | (39) | |
| Gsy2 Glycogen Synthase |
In vitro kinase assay with Psk2 | (39) | |
|
| |||
| H. sapiens | Gsy Glycogen Synthase |
In vitro kinase assay with hPASK Copurification |
(38) |
| eEF1A1 Eukaryotic Translation Elongation Factor |
Yeast and mammalian two-hybrid Copurification In vitro kinase assay with hPASK Colocalization |
(40) | |
| S6 Ribosomal Protein S6 |
In vitro kinase assay with hPASK In vivo phosphorylation assay |
(27) | |
| Pdx-1 Pancreatic Duodenal Homeobox-1 |
In vitro kinase assay with hPASK | (29) | |
The protein abbreviation and description are given for each substrate.