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. 1999 Mar 15;103(6):R15–R21. doi: 10.1172/JCI6179

Figure 1.

Figure 1

Nondenaturing gradient gel electrophoresis followed by Western blot analysis with goat anti–apo E antibody reveals that apo E in human CSF and apo E secreted by astrocytes are in HDL-like particles of overlapping size, mostly between 10 and 17 nm. Lane 1, pooled human CSF (20 μl) containing 100 ng apo E. Lane 2, pooled human CSF (30 μl) containing 150 ng apo E. Lane 3, astrocyte-conditioned media (20 μl) containing 100 ng apo E4 derived from GFAP–apo E4+/– astrocytes after 3 days in culture and collected as described (21). Lane 4, astrocyte-conditioned media (20 μl) from GFAP–apo E–/– cells. Size markers (Pharmacia Biotech Inc., Piscataway, New Jersey, USA) are expressed in nanometers. CSF, cerebrospinal fluid; GFAP, glial fibrillary acidic protein; HDL, high-density lipoprotein.