Figure 2.

IFN-γ increases IP-10/CXCL10 levels in orbital fibroblasts. Primary cultures of orbital fibroblasts (5 × 10⁴) of patients with TAO (n=2) and a subject without TAO (n=1) were incubated with IFN-γ (10 ng ml⁻¹), LPS (100 ng ml⁻¹) and IL-1β (10 ng ml⁻¹) for 24 h and H₂O₂ (100 μM) for 30 min. After treatment, supernatants were collected and IP-10/CXCL10 protein levels in each culture supernatant were measured by ELISA. Data are presented as the fold increases in relative IP-10/CXCL10 levels from orbital fibroblasts compared with each control. The graph shows the mean±s.d. of three independent replicates (*P<0.05 vs each untreated control).