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. 2014 Jul 3;9(7):e101220. doi: 10.1371/journal.pone.0101220

Figure 4. Effect of the diatom PUAs 2-trans,4-trans-decadienal (DD), 2-trans,4-trans-octadienal (OD) and 2-trans,4-trans-heptadienal (HD) on the human lung adenocarcinoma cell line A549.

Figure 4

Control and treated cells double stained with acridine orange and ethidium bromide after 48 µM DD, OD and HD observed at the confocal microscope. Numbers indicate (1) normal cells; (2) early apoptotic cells; (3) late apoptotic cells; (4) necrotic cells (see material and methods for details). Arrows indicate cells with fragmented nuclei.