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. 2014 Jun 23;29(6):740–749. doi: 10.1016/j.devcel.2014.05.001

Figure 1.

Figure 1

H4K20 Monomethylation Is Detected in Centromeres

(A) ChIP-seq analysis with anti-CENP-A or anti-H4K20me1 antibodies on chromosome Z in DT40 cells. Sequence reads were mapped at 10 kb windows. Position of a major peak for H4K20me1 was identical to that for CENP-A.

(B) High-resolution profile of ChIP-seq analysis for CENP-A (green) or H4K20me1 (magenta) around centromere region of chromosome Z (42.62–42.66 Mb region of chicken chromosome Z). Both profiles overlap (gray).

(C) ChIP-seq analysis with anti-CENP-A (green) or anti-H4K20me1 (magenta) antibodies in neocentromere-containing DT40 cell lines (After #BM23 and #0514). ChIP-seq data at these neocentromere loci in parental Z3 cell line are also shown (before).

(D) Immunofluorescence analysis with Cy3-labeled anti-H4K20me1 antibodies (red) in mitotic chromosomes in chicken DT40 cells expressing CENP-A-GFP (green). Colocalization of H4K20me1 with CENP-A was observed (merge). Scale bar, 5 μm.

(E) Immunofluorescence analysis with Cy3-labeled anti-H4K20me1 antibodies (red) in mitotic chromosomes in human HeLa cells expressing CENP-A-GFP (green). Colocalization of H4K20me1 with CENP-A was observed (merge). Scale bar, 5 μm.