Figure S3.

Map and Model Quality, Related to Figure 1

(A) Gold-standard Fourier Shell Correlation (FSC) curve for the map used for building of the 60S subunit (60S-mask, Map 2), where the resolution is demarcated using the FSC = 0.143 criterion.

(B) FSC curves of the final model versus the full map used in A (black); of a model refined in the first of two independent halves of the map (red); and of that same model versus the second independent map, which was not used for refinement (blue). The vertical dashed line indicates the highest resolution used in these model refinements.

(C and D) As in (A) and (B) but for the 40S subunit built using Map 5. The final refined models for both the 40S and 60S subunits were rigid-body fit into the density for each of the other classes of particles.

(E and F) FSC curves for maps of the remaining classes.