Figure S5.

Density in Different Regions of the idle Sec61 Structure, Related to Figure 4

(A) Displayed here are two views of a density map filtered to 4.2 Å resolution that demonstrates the overall fit of the idle Sec61 channel. (B) Representative density for the N terminus of Sec61γ (tan), which was sufficiently ordered to allow placement of amino acid side chains. (C) Displayed is an unsharpened density map, in which the detergent micelle has been removed using Chimera (Goddard et al., 2007), visualized from the lumenal side of the channel to show placement of the backbone of the plug in the idle channel.