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. 2014 Jul 3;9(7):e101233. doi: 10.1371/journal.pone.0101233

Figure 7. Newly synthesised extractable type II collagen in loaded and unloaded discs.

Figure 7

NP tissue of loaded (L) and un-loaded (UL) IVDs were analysed by western blotting after 14 days of culture. The IVDs were injected 100 µg trypsin prior to loading. A, Semi-quantitative analysis of type II collagen with a molecular weight of about 135 kDa. Each sample was evaluated in duplicate on 2 separate western blots. Band intensity was normalized to reference samples (R) included on each blot. B, representative western blot of pooled extracts. (n 6 discs from different animals, p<0.0001). Error bars represent SEM.