Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jun 10;10(7):664–676. doi: 10.7150/ijbs.8232

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

PMC Copyright notice

URHC regulates cell proliferation and apoptosis by down-regulating ZAK. (A) The proliferation of SMMC7721 cells that had been transfected with URHC-siRNA, ZAK-siRNA, URHC-siRNA plus ZAK-siRNA, or the siRNA control was examined at 48 h using the MTT assay. (B) SMMC7721 cells were transfected as described in (A), and the cell cycle distribution was calculated by flow cytometer after staining with PI. (C) The protein expression levels of Bax, Bcl-2, caspase 3 and cleaved caspase 3 were measured using western blot analyses, and the ratios of Bax/Bcl-2 (D) and cleaved caspase 3/caspase 3 (E) were determined. β-actin was used as a reference. The results show the means ± SD from at least 3 separate experiments. *, P < 0.05; **, P < 0.001; NS, nonsignificant.