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. 2014 Jun 10;10(7):664–676. doi: 10.7150/ijbs.8232

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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URHC regulates MAPK activity through ZAK. (A) The expression and phosphorylation of ERK, JNK, and p38 were examined using western blot analyses in SMMC7721 cells that had been transfected with URHC-siRNA or the siRNA control. (B) The expression and quantitative analyses of phosphorylation of ERK (C), JNK (D), and p38 (E) were examined at 48 h using western blot analyses in SMMC7721 cells that had been transfected with URHC-siRNA, ZAK-siRNA, URHC-siRNA plus ZAK-siRNA, or the siRNA control. β-actin was used as a reference. The results show the means ± SD from 3 separate experiments. *, P < 0.05; **, P < 0.001.