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. 2014 Jul 3;10(7):e1004233. doi: 10.1371/journal.ppat.1004233

Figure 2. Systemic division of inflationary CD8s.

Figure 2

C57BL/6 mice were infected with K181 MCMV for more than 3 months before organs were harvested for lymphocyte analyses. Mice were injected i.p. with 1 mg BrdU 16 hours before sacrifice. (A) Shown is the average frequency of Ki67 and BrdU co-stained M38-specific (left), IE3-specific (middle) and M45 (right) cells in the indicated organs 16 hours after BrdU injection. Error bars represent the standard error of the mean (n = 7). Statistical significance was measured by a paired student's t-test (*p<.05, **p<.01). (B) Number and (C) phenotype of Ki67-expressing T cells specific for M38 (left), IE3 (middle) and M45 (right), in the indicated organs. Error bars represent the standard error of the mean. Data are combined from 3 independent experiments and 12 animals. (D) Spread defective MCMV induces a similar pattern of division as wild type MCMV. C57BL/6 mice were infected with ΔgL-MCMV for more than 3 months and then injected with BrdU as in “A”. Data are displayed as in “A” and combined from two independent experiments (n = 8) Statistical significance was measured by a paired student's t-test (* p<.05).