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. 2014 Jul 3;10(7):e1004233. doi: 10.1371/journal.ppat.1004233

Figure 5. Most inflationary CD8s are exposed to the blood supply.

Figure 5

Mice infected with K181 MCMV for more than 3 months were injected with fluorochrome labeled anti-CD8α antibody to identify T cells exposed to the blood supply. After perfusion and processing the organs, cells were counterstained with anti-CD8β, to identify all CD8 T cells. (A) Shown is representative FACS staining with the injected antibody for all CD8s (top), M38-specific CD8s (middle), and dividing effector-phenotype M38-specific CD8s (bottom) in the blood, spleen, liver, lung and mediastinal lymph nodes. (B) Graph shows mean frequency of cells labeled by i.v. staining for all CD8s, M38-specific CD8s, and dividing effector-phenotype M38-specific CD8s for the indicated organs. Data are pooled from 4 independent experiments (n = 15) and representative of seven independent experiments. Statistical significance was measured by paired student's t-tests (*p<.05, **p<.01, ***p<.001, **** p<.0001). Error bars represent the standard error of the mean.