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. 2014 Jul 3;10(7):e1004216. doi: 10.1371/journal.ppat.1004216

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© 2014 Li et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Lung sections were examined by immunohistochemistry (IHC) using monoclonal antibodies (SDOW17) specific for the N protein of PRRSV, and numbers of positive cells in lungs were scored. Representative pictures of immunohistochemistry examinations and mean scores of lungs of the dead piglets during the experiment (A) and of euthanized piglets by the end of experiment (B) in each group are shown. The macrophages stain intensely dark brown for the PRRSV antigen. Hollow arrow indicates positive signals in macrophages within or around alveolus and bronchus. Asterisk indicates a significant difference in IHC scores between RvHB-1/3.9 and RvHJn9n10n11 (*P<0.05).