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. 2014 Jul 3;10(7):e1004188. doi: 10.1371/journal.ppat.1004188

Figure 5. Induction of necrotic cell death and the release of bacteria and IL-1β in P2X7R−/− and C57BL/6 BMDMs infected with hypervirulent mycobacteria.

Figure 5

C57BL/6 and P2X7R−/− BMDMs were infected with H37Rv Mtb, Beijing 1471 Mtb and MP287/03 Mbv at an MOI of 10. (A) On day 4 p.i., necrotic cells were identified by AO/EthBr incorporation. (B) Images show C57BL/6 and P2X7R−/− BMDMs on day 4 p.i. with Beijing 1471 Mtb (200× magnification; bar scales correspond to 100 µm). (C) The release of mycobacteria was examined by CFU quantification in the supernatants of the infected cultures described in A. (D) On day 3 p.i., IL-1β was quantified in the culture supernatants. Significant differences were observed for the indicated experimental conditions (***p<0.001). The data represent the means ± SD of samples in triplicate. The data are representative of three separate experiments.