Table 1. Strains and plasmids used in this study.
| Name | Characteristics | Reference |
| Strains | ||
| DH5α | E. coli | Laboratory stock |
| AAG1 | E. coli, MG1655 ΔlacZ | [89] |
| J99 | H. pylori clinical isolate, USA, Duodenal ulcer | [91] |
| J99StrR | Streptomycin resistant variant of J99, mouse-adapted | This study |
| 26695 | H. pylori clinical isolate, UK, Gastritis | [54] |
| G27 | H. pylori clinical isolate, Italy, Gastritis | [92] |
| 17875/sLex | H. pylori clinical isolate, Australia, Gastritis | [93] |
| SMI109 | H. pylori clinical isolate, Sweden, Gastric cancer | [13] |
| SMI109 ΔsabA | ΔsabA::kan variant of SMI109 | This study |
| SMI109 ΔsabA::rpsLCAT | sabA promoter in SMI109 replaced by rpsLCAT cassette | This study |
| SMI109 T-variants | Constructed variants of SMI109 with different repeat tract length in sabA promoter; T1–T21 and A13, C13 | This study |
| SMI109 pyrG::lacZ | SMI109 harboring pAAG202 on the chromosome, KmR | This study |
| SMI109 pyrGΔ5::lacZ | SMI109 harboring pAAG203 on the chromosome, KmR | This study |
| SMI109 hp_0350::lacZ | SMI109 harboring pAAG204 on the chromosome, KmR | This study |
| SMI109 hp_0350Δ5::lacZ | SMI109 harboring pAAG205 on the chromosome, KmR | This study |
| SMI109 Δhup | Δhup::kan variant of SMI109 | This study |
| SMI109 ΔnapA | ΔnapA::kan variant of SMI109 | This study |
| Plasmids | ||
| pCR TOPO | General cloning vector, CbR | Life Technologies |
| pUC19 | General cloning vector, CbR | [94] |
| pRZ5202 | lacZ promoter fusion vector for E. coli, CbR | [95] |
| pBW | lacZ promoter fusion vector for H. pylori, KmR | [96] |
| pAAG132 | sabA promoter from 26695 cloned in pRZ5202, CbR | This study |
| pAAG134 | sabA promoter from J99 cloned in pRZ5202, CbR | This study |
| pAAG135 | sabA promoter from G27 cloned in pRZ5202, CbR | This study |
| pAAG136 | sabA promoter from 17875/sLex cloned in pRZ5202, CbR | This study |
| pAAG107 | sabA promoter from SMI109 cloned in pRZ5202, CbR | This study |
| pAAGXX* | Variants of pAAG107 with different length of repeat tract in sabA promoter; T1–T21 and A13, C13 | This study |
| pAAG198-201 | Δ46 variants of PsabA::lacZ, with different length of repeat tract in sabA promoter; T9, T13, T18 and A13 | This study |
| pAAG206-208 | Scrambled UP-like elements of PsabA::lacZ | This study |
| pAAG202 | pyrG promoter from SMI109 cloned in pBW, KmR | This study |
| pAAG203 | pyrG promoter from SMI109, lacking 5T's, cloned in pBW, KmR | This study |
| pAAG204 | hp_0350 promoter from SMI109 cloned in pBW, KmR | This study |
| pAAG205 | hp_0350 promoter from SMI109, lacking 5A's, cloned in pBW, KmR | This study |
| pAAG178 | Δhup::kan construct cloned in pUC19, KmR, CbR | This study |
| pBlue+ΔnapA | ΔnapA::kan construct cloned in pBlueScript, KmR, CbR | [78] |
| pKD4 | Used as template for kanamycin resistance cassette | [97] |
* Several plasmids with sabA::lacZ transcriptional fusion, with different length of the T-tract, were used.