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. 2014 Jul 3;10(7):e1004222. doi: 10.1371/journal.ppat.1004222

Figure 1. A conserved Legionella-effector PI4P-binding region.

Figure 1

(A) Schematic representation of the effector proteins DrrA, Lpg1101 and Lpg2603. Indicated are regions correspond to the ATase (blue), GEF (yellow) and PI4P-binding domain (green) of DrrA. The C-terminal regions of Lpg1101 and Lpg2603 (green) have homology to the PI4P-binding region in DrrA. Non-homologous regions of unknown function in Lpg1101 (brown) and Lpg2603 (pink) are indicated. (B) Alignment of the LEPR regions in DrrA, Lpg1101 and Lpg2603 reveals conserved amino acid residues corresponding to the PI4P-binding pocket of DrrA (grey). (C) Binding of His-tagged DrrA, Lpg1101 and Lpg2603 to lipids immobilized on nitrocellulose membranes was detected using an anti-His antibody. Template shows the location of specific lipids. All three proteins showed specific binding to the spot containing PI4P. DAG, Diacylglycerol; PA, phosphatidic acid; PS, phosphatidyl serine; PE, phosphatidylethanolamine; PC, phosphatidylcholine; PG, phosphatidylglycerol; PtdIns, phosphatidylinositol; PtdIns(4,5)P2, phosphatidylinositol 4,5 di-phosphate; PtdIns(3,4,5)P3, phosphatidylinositol 3,4,5 tri-phosphate.