Figure 3. Role of the Membrane Insertion Motif (MIM) in LEPR-containing effectors association with the PM.
(A) Alignment of amino acid region containing the exposed leucine residues of the MIM motif from DrrA with the similar region from the LEPR-domain containing effectors Lpg1101 and Lpg2603. The key exposed residues are indicated with arrows. (B) Z-stack micrographs of confocal images of HEK293 cells expressing GFP or EYFP-tagged fluorescent LEPR effectors and exposed leucine* to alanine mutants (*wild type exposed residues shown in red).