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. 2014 Jul 4;4:5574. doi: 10.1038/srep05574

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(a) Schematic of the wide-field time-gated fluorescence microscope. BF: bandpass filter, BS: beam splitter, DM: dichroic mirror, ICCD: intensified charge-coupled device, L: lens, M: mirror, Obj: objective, PC: personal computer, and PD: photodiode. The drawing was created with Microsoft Office PowerPoint 2007. (b) Power dependence of the second-order Stokes Raman conversion of the 532-nm laser using a Ba(NO₃)₂ crystal as the Raman shifter. The maximum output energy is about 25 μJ/pulse. (c) Oscilloscope traces of the laser pulses before and after passing through the Raman shifter.