TABLE 1.
Single channel measurements of B. burgdorferi P13 in different KCl concentrations and different electrolytes
The P13 conductance (G) in each salt solution was taken from the highest conductance value observed in a Gaussian distribution of single channel conductance. To analyze P13 conductance, in each case at least 100 channels were reconstituted in DPhPC membranes at 20 mV voltage. The solutions were used unbuffered with a pH close to 6 unless otherwise indicated. The conductivity of each salt solution (χ) was measured at room temperature with a conductometer (Knick 703). The ion activities (γ) of the salts at 25 °C are given in parentheses (51).
| Electrolyte | Concentration (γ) | χ | P13 G |
|---|---|---|---|
| m | mS/cm | nS | |
| KCl | 0.1 (0.77) | 13.1 | 0.05 |
| 0.3 (0.68) | 37.1 | 0.075 | |
| 1 (0.60) | 106.5 | 0.6 | |
| 3 (0.56) | 291.8 | 1.5 | |
| LiCl | 1 (0.77) | 70.3 | 0.2 |
| KCH3COO (pH 7) | 1 (0.78) | 68.6 | 0.25 |