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. 2014 May 12;289(27):18641–18656. doi: 10.1074/jbc.M113.538777

FIGURE 1.

FIGURE 1.

PLZF-RARα promotes cell proliferation but does not induce apoptosis. A, FACS analysis of HEK293 and HCT116 cells transfected with either a pcDNA3 or pSG5-PLZF-RARα plasmid. B, MTT assay of cell proliferation. HEK293 and HCT116 cells transfected with either a pcDNA3 or pSG5-PLZF-RARα plasmid were grown for 1–4 days and analyzed for MTT to formazan conversion using colorimetry at 540–600 nm. *, p < 0.05. Mean values of three independent experiments are shown. Error bars are too small to see and represent S.D. C, FACS analysis of apoptosis. HEK293 cells were transfected with either a pcDNA3 or pSG5-PLZF-RARα plasmid. The cells were stained with Annexin V and propidium iodide 48 h after transfection. x axis, Annexin V-FITC staining; y axis, propidium iodide (PI) staining. The numbers shown in the two right quadrants represent the percentage of cells undergoing apoptosis.