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. 2014 May 12;289(27):18641–18656. doi: 10.1074/jbc.M113.538777

FIGURE 2.

FIGURE 2.

PLZF-RARα represses the transcription of p53 pathway genes in HEK293 cells. A, transient transcription assays for the ARF, MDM2, TP53, and CDKN1A genes of the p53 pathway. The PLZF-RARα expression vector and promoter-luciferase fusion reporter plasmid were transiently co-transfected into HEK293 cells, and luciferase activity was measured. The results are the average of three independent assays. Bars, standard deviations. B and C, RT-qPCR and Western blot (WB) analyses showing PLZF-RARα and endogenous MDM2, TP53, and CDKN1A expression in HEK293 cells transfected with a PLZF-RARα expression vector. GAPDH, control. D, structures of the four CDKN1A promoter constructs tested and transient transcription assays for CDKN1A gene expression. The PLZF-RARα expression vector and various CDKN1A promoter-luciferase fusion reporter plasmids (shown on the left) were transiently co-transfected into HEK293 cells and luciferase activity was measured. *, p < 0.05; t test.