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. 2014 May 12;289(27):18641–18656. doi: 10.1074/jbc.M113.538777

FIGURE 4.

FIGURE 4.

Transcription repression of CDKN1A by PLZF-RARα can be independent of p53. A, transcription assays. HCT116 p53+/+ and p53−/− cells were transiently co-transfected with a PLZF-RARα expression vector and a pGL2-CDKN1A-Luc (−2.3 kb) reporter plasmid, after which the cells were treated with etoposide and analyzed for luciferase activity. B, MTT assay of cell proliferation. HCT116 p53−/− cells transfected with either a pSG5 or pSG5-PLZF-RARα plasmid were grown for 1–4 days and analyzed for the MTT to formazan conversion using colorimetry at 540–600 nm. C–F, RT-qPCR and Western blot (WB) analyses showing PLZF-RARα and endogenous p53 and p21 expression in the HCT116 p53−/− cells transfected with the PLZF-RARα expression vector. GAPDH, control. *, p < 0.05; N.S., not significant; t test.