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Size exclusion chromatography shows that PlaB forms homodimers. Size exclusion chromatogram of 500 μl, 1 mg/ml (18.6 μm), purified Strep-PlaB expressed from E. coli BL21 (pKK19) (A), and calibration curve (B) (Ve = elution volume of standard protein or PlaB; Vo = void volume of 8 ml) are shown. Results are representative of at least two independent experiments. mAU, milliabsorbance units.
