FIGURE 6.

C-terminal 15-amino acid region of PlaB is not essential for oligomerization. Regularized SAXS curves determined for Strep-PlaB (WT) and the three C-terminally truncated variants lacking 5, 10, or 15 amino acids (WT-5aa, WT-10aa, and WT-15aa). The logarithm of scattering intensity is plotted versus the momentum transfer s = 4πsinθ/λ and the corresponding resolution d_min. The curves coincide well on the left-hand side of the diagram, which corresponds to low values of momentum transfer and indicates identical low- resolution structures of rStrep-PlaB and variants, i.e. the same oligomeric states. Deviations of the curves on the right-hand side result from minor structural differences apparent only at higher resolution.