TABLE 1.

Overview of strains used in this study

The abbreviations used are as follows: term., terminal; aa, amino acid; L.pn, L. pneumophila Corby.

Organism	Mutation(s)/plasmid	Tags	Selection marker	Source/Ref.
L.pn.	Wild type L. pneumophila Sg1 Corby			25
L.pn.	dotA knock-out E01		KmR	This studya
L.pn. plaB−	plaB::km insertion mutant (= plaB1)		KmR	14
L.pn. plaB−	pJB04 = pBCKS plaB (complementing strain)		CmR	13
L.pn. plaB−	pKK50 = pBCKS plaB (aa 1–459)		CmR	This study
L.pn. plaB−	pJB12 = pBCKS plaB D203N		CmR	13
L.pn. plaB−	pJB06 = pBCKS plaB S85A		CmR	13
E. coli BL21	pKK19 = pGP172 plaB	N-term. Strep tag	AmpR	This study
E. coli BL21	pKK25 = pGP172 plaB (aa 1–469)	N-term. Strep tag	AmpR	This study
E. coli BL21	pKK26 = pGP172 plaB (aa 1–464)	N-term. Strep tag	AmpR	This study
E. coli BL21	pKK27 = pGP172 plaB (aa 1–459)	N-term. Strep tag	AmpR	This study

^a P. Aurass, S. Tröller, and A. Flieger, unpublished data.