TABLE 2.
Overview of primers used in this study
The abbreviations used are as follows: term., terminal; aa, amino acid.
| Plasmid | Gene | Tag | Primer name | Primer sequence (5′ to 3′) |
|---|---|---|---|---|
| pKK19 | plaB | N-term. Strep tag | plaBkpnIf | -CAGGGTACCATGATTGTTATC- |
| plaB_SmaIr | -CTACCCGGGTCAATCTATCTTT- | |||
| pGP172XmaIf | -CTACCCGGGGATCCGGCT- | |||
| pGP172KpnIr | -GATGGTACCGGCGCCTTTTTCG- | |||
| pKK25 | plaB (aa 1–469) | N-term. Strep tag | pGP172plaB-5f | -GAAAGCCAACTTGACCCGGGGATCCGGC- |
| pGP172plaB-5r | -CCGGGTCAAGTTGGCTTTCCACTGATTTTTGCCGGAG- | |||
| pKK26 | plaB (aa 1–464) | N-term. Strep tag | pGP172plaB-10f | -GCAAAAATCTGACCCGGGGATCCGGC- |
| pGP172plaB-10r | -CCGGGTCAGATTTTTGCCGGAGTAAGGTTGTTGC- | |||
| pKK27 | plaB (aa 1–459) | N-term. Strep tag | pGP172plaB-15f | -CAACAACCTTTGACCCGGGGATCCGGC- |
| pGP172plaB-15r | -CCGGGTCAAAGGTTGTTGCTGATACGGAAAACTGTCC- | |||
| pKK50 | plaB (aa 1–459) | pJB04–15Ctf | -CAACAACCTTTGATACCTCTTTCGTTTGATGC- | |
| pJB04–15Ctr | -GAGGTATCAAAGGTTGTTGCTGATACGGAAAACTG- |